Nuclear Lamin A C

Isoform lamin c does not undergo posttranslational modifications.

Nuclear lamin a c. Performed under reducing conditions. The last 15 amino. Lamin a c is a major constituent of the nuclear lamina a thin filamentous protein layer that lies beneath the nuclear envelope. During mitosis the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated.

Lamin proteins are thought to be involved in nuclear stability chromatin structure and gene expression. Hela whole cell lysate lysates proteins at 20 µg per lane. The cleavage of lamins results in nuclear dysregulation and cell death 5 6. Lamin a c knockout hap1 whole cell lysate lane 3.

Lamin structure and assembly. Vertebrate lamins consist of two types a and b. In mammals alternative splicing gives rise to lamin a and lamin c and the less abundant isoforms lamin aδ10 and lamin c2 fisher et al 1986. Machiels et al 1996 a type lamins are absent in early embryonic cells but are present in.

The nuclear lamina consists of two components lamins and nuclear lamin associated membrane proteins. Prelamin a undergoes one additional processing step. Wild type hap1 whole cell lysate lane 2. Prelamin a the precursor to mature lamin a lamin b1 and lamin b2 contain a carboxyl terminal caax motif that triggers farnesylation and methylation of a carboxyl terminal cysteine.

Furukawa et al 1994. The lamins are type v intermediate filaments which can be categorized as either a type lamin a c or b type lamin b 1 b 2 according to homology of their dna sequences biochemical properties and cellular localization during the cell cycle. During apoptosis lamin a c is specifically cleaved into a large 41 50 kda and a small 28 kda fragment 3 4. 9 13 some studies have demonstrated that lamins a and c are not required for the formation of the nuclear lamina yet disruptions in the lmna gene can contribute.

Anti lamin a lamin c antibody epr4100 nuclear envelope marker ab108595 at 1 10000 dilution lane 1. Emd is required for proper localization of non farnesylated prelamin a c. Lamin a c is cleaved by caspase 6 and serves as a marker for caspase 6 activation. I personally use the abcam histone h3 c terminal rabbit polyclonal ab1791.

2000 j struct biol 129 313 23. Through alternate splicing this gene encodes three type a lamin isoforms. A type lamins are encoded by a single gene lmna. Lamins are grouped into a type and b type lamins.

Farnesylation of prelamin a c facilitates nuclear envelope targeting and subsequent cleaveage by zmpste24 face1 to remove the farnesyl group produces mature lamin a c which can then be inserted into the nuclear lamina. The very first modification involving farnesylation of prelamin a is crucial to the development of mature lamin a.