Nuclear Envelope Lamin

Taken together the levels of nuclear lamin b expression affect extracellular trap formation in a negative dose dependent manner.

Nuclear envelope lamin. Anti lamin b1 antibody nuclear envelope marker ab16048 at 1 1000 dilution human pancreatic cell line whole cell lysate at 20 µg secondary hrp conjugated goat anti rabbit antibody at 1 2000 dilution developed using the ecl technique. Flow cytometry anti lamin b1 antibody 119d5 f1 nuclear envelope marker ab8982 overlay histogram showing hela cells stained with ab8982 red line. Mutations in lamin genes can result in several genetic laminopathies which may be life threatening. Disassembly of the nuclear envelope and lamina occurs at prometaphase a period of the mitotic cell cycle that precedes chromosome segregation.

The nuclear envelope fragments into membrane vesicles and the lamin filaments are disassembled into lamin dimers. Transcription factors that bind to the lamina include the retinoblastoma transcriptional regulator rb germ cell less gcl sterol response element binding protein srebp1. Therefore our results demonstrated that nuclear lamin b is a substantial component of the nuclear envelope in neutrophils similar to other cell types goldberg et al 2008. Decreasing lamin b phosphorylation by pkcα inhibition genetic deletion or by mutating the pkcα consensus sites on lamin b attenuates extracellular trap formation.

Performed under reducing conditions. Furthermore we demonstrate that nuclear translocation of pkcα which serves as the kinase to induce lamin b phosphorylation and disassembly results in nuclear envelope rupture. Lamin b1 plays an important role in the nuclear envelope stability the regulation of gene expression and neural development. Nuclear envelope proteins that are bound to the lamina include nesprin emerin lamina associated proteins 1 and 2 lap1 and lap2 the lamin b receptor lbr and man1.

Duplication of lmnb1 or missense mutations increasing lmnb1 expression are associated with autosomal dominant leukodystrophy. The cells were fixed with 100 methanol 5 min and then permeabilized with 0 1 pbs tween for 20 min.